ASEB: A Web Server for KAT-specific Acetylation Site Prediction



Tutorial for prediction process help.png


Please try to click the buttons on this tutorial page!

Predefine:
CBP/p300      GCN5/PCAF      TIP60/MYST1/2/3/4     HDAC1/HDAC2/HDAC3     SIRT1

These five radio buttons are used to select a KAT or HDAC family.

Swiss-Prot accession number (recommended) or protein name:


The textbox above is used to input the Swiss-Prot accession number for a query protein.
The Load Sequence button is used to query the sequence from the server using the Swiss-Prot accession number entered.
If users fail to load the sequence, then please input the sequence directly.
Try to click the Example button, and click the Load Sequence button.

Sequence:



The textbox above is used to input a query protein sequence (once users fail to load sequence using the Swiss-Prot accession number).
The Submit button is used to submit the query protein sequence to the server. Try to click it.
The Clear button is used to clear all the inputs and outputs. Try to click it.
To see the sample output, try to click the Example button, and click the Submit button.

The shortest path between the lysine-acetyl-transferase and query protein:

CBP (CREBBP) PINA CREBBP -> TP53 network view all shortest pathways
CBP (CREBBP) STRING CREBBP -> TP53 network view all shortest pathways
p300 (EP300) PINA EP300 -> TP53 network view all shortest pathways
p300 (EP300) STRING EP300 -> TP53 network view all shortest pathways

This table shows the shortest path between the lysine-acetyl-transferase and the putative substrate.
The first column indicates the lysine-acetyl-transferase.
The second column indicates the database used to search the shortest path.
The third column shows the path in details.
The fourth column presents a link that is used to show one of the shortest path graphically.
The last column shows all the shortest pathways.
Try to click the links in each column.

ASEB P-values for candidate sites:

KAT Site Sequence P-value
CBP/p300 24 QETFSDLWKLLPENNVL 0.7536
CBP/p300 101 LSSSVPSQKTYQGSYGF 0.247
CBP/p300 120 GFLHSGTAKSVTCTYSP 0.0178
CBP/p300 132 CTYSPALNKMFCQLAKT 0.7102
CBP/p300 139 NKMFCQLAKTCPVQLWV 0.9734
CBP/p300 164 RVRAMAIYKQSQHMTEV 0.0661
CBP/p300 291 RTEEENLRKKGEPHHEL 0.4589
CBP/p300 292 TEEENLRKKGEPHHELP 0.007
CBP/p300 305 HELPPGSTKRALPNNTS 0.015
CBP/p300 319 NTSSSPQPKKKPLDGEY 0.0001
CBP/p300 320 TSSSPQPKKKPLDGEYF 0.017
CBP/p300 321 SSSPQPKKKPLDGEYFT 0.491
CBP/p300 351 ELNEALELKDAQAGKEP 0.8597
CBP/p300 357 ELKDAQAGKEPGGSRAH 0.1173
CBP/p300 370 SRAHSSHLKSKKGQSTS 0.001
CBP/p300 372 AHSSHLKSKKGQSTSRH 0.003
CBP/p300 373 HSSHLKSKKGQSTSRHK 0.001
CBP/p300 381 KGQSTSRHKKLMFKTEG 0.001
CBP/p300 382 GQSTSRHKKLMFKTEGP 0.001
CBP/p300 386 SRHKKLMFKTEGPDSD- 0.04

1) Sequences with less than 8 amino acids on each side surrounding K are filled by dashes.
2) The P-values for the query peptides are between 0.0001 and 1, with a minimum interval of 0.0001. A lower P-value indicates a higher probability of the acetylation by the selected KAT family.
3) Once a P-value is given, its rank from the background will be shown. The background P-values were calculated from all lysine sites on human proteins and then ranked from lowest to highest. Lysine sites with P-values lower than the top 10% are highlighted. We suggest users pay more attention to these sites.

This table shows the ASEB P-values for the candidate sites.
The first column indicates the lysine-acetyl-transferase family.
The second column indicates the position of a specific site.
The third column shows the lysine site and its surrounding amino acids.
The last column shows the ASEB P-value for this site.
If users click on a P-value, a plot will appear and show the rank of the generated P-value from the background P-values for all lysine sites on human proteins. Try to click it.